TY  - JOUR
T1  - A Comparative Evaluation of Colonic Microflora Profiles and Plasma Metabolites in Unilateral Ureteral Obstruction–Related Chronic Kidney Disease Following Intervention with the Chinese Medicine FuZhengHuaYuJiangZhuTongLuo versus AST-120
A1  - Mohammed Rahman
A1  - Abdul Karim
A1  - Hasan Mahmud
JF  - Interdisciplinary Research in Medical Sciences Specialty
JO  - Interdiscip Res Med Sci Spec
SN  - 3062-4401
Y1  - 2023
VL  - 3
IS  - 1
DO  - 10.51847/t1GcnyFCQ3
SP  - 146
EP  - 163
N2  - Extensive research has explored how Chinese herbal formulations may slow the advancement of chronic kidney disease (CKD) by modulating colonic microflora and microbiota-derived metabolites. Despite this, it remains unclear whether FuZhengHuaYuJiangZhuTongLuo (FZHY) exerts regulatory effects on CKD that parallel those reported for AST-120.To address this question, we systematically compared the influence of FZHY and AST-120 on gut microbiota composition and plasma metabolomic profiles in a chronic kidney disease model. A unilateral ureteral obstruction (UUO)-induced CKD rat model was established, after which the animals received either FZHY or AST-120. Plasma, stool, and kidney samples were subsequently evaluated using non-targeted LC-MS metabolomics, 16S rRNA sequencing, and histopathological assessment. Correlation networks linking bacterial candidates with key metabolites were also constructed. Both interventions markedly mitigated UUO-associated renal dysfunction and fibrosis while reshaping microbial communities and metabolic signatures. Relative to the untreated UUO group, FZHY administration increased the abundance of p_Firmicutes and o_Peptostreptococcales_Tissierellales, accompanied by the upregulation of 14 negative-ion and 40 positive-ion metabolites and the downregulation of 21 and 63 metabolites, respectively. In contrast, AST-120 supplementation elevated g_Prevotellaceae_NK3B31_group and f_Prevotellaceae, and altered 12 negative-ion metabolites upward and 23 downward, along with 56 positive-ion metabolites upregulated and 63 downregulated. Furthermore, FZHY enhanced bacterial biomarkers that showed negative correlations with harmful metabolites such as 4-hydroxyretinoic acid and positive correlations with beneficial ones like L-arginine. AST-120 enriched bacterial biomarkers inversely associated with toxic compounds including glycoursodeoxycholic acid, 4-ethylphenol, and indole-3-acetic acid.Both FZHY and AST-120 alleviated renal injury in the UUO-induced CKD model, potentially through restoration of disrupted microbial and metabolic pathways. Because each treatment modulated distinct bacterial taxa and metabolites, FZHY may serve as a valuable complementary therapeutic strategy alongside AST-120 in CKD management.
UR  - https://galaxypub.co/article/a-comparative-evaluation-of-colonic-microflora-profiles-and-plasma-metabolites-in-unilateral-uretera-j9bxmfg854uxusm
ER  -