TY  - JOUR
T1  - Assessment of Leishmanicidal Properties and Cell Toxicity of the Hydroalcoholic Extract from Thymus kotschyanus In Vitro
A1  - Claire Dubois
A1  - Antoine Girard
A1  - Sophie Bernard
JF  - Specialty Journal of Pharmacognosy, Phytochemistry, and Biotechnology
JO  - Spec J Pharmacogn Phytochem Biotechnol
SN  - 3062-441X
Y1  - 2023
VL  - 3
IS  - 1
DO  - 10.51847/9NTGBEZ3Ta
SP  - 224
EP  - 231
N2  - Existing therapies for leishmaniasis face challenges including adverse reactions, reduced effectiveness, and emerging resistance. Plants from the Thymus genus are recognized for their therapeutic properties. This research investigated the potential antileishmanial activity and toxic effects of a hydroalcoholic extract derived from Thymus kotschyanus through laboratory-based tests. The above-ground portions of T. kotschyanus were processed via maceration using 70% ethanol to obtain the extract. Its impact was assessed on both promastigote and amastigote forms of Leishmania major by enumerating parasites directly within a macrophage system. Toxicity was measured using the MTT method. Rates of macrophage infection and counts of intracellular amastigotes were compared against untreated controls and glucantime-treated groups (standard drug). The extract suppressed proliferation of promastigotes and amastigotes, with peak inhibition at 48 hours. IC50 values against non-infected macrophages reached 381.8 μg/L at 48 hours and 392.9 μg/L at 72 hours. Doses of 300 and 350 μg/mL markedly lowered the proportion of infected macrophages (p=0.042 and p=0.001, respectively) and the quantity of amastigotes inside macrophages (p
UR  - https://galaxypub.co/article/assessment-of-leishmanicidal-properties-and-cell-toxicity-of-the-hydroalcoholic-extract-from-thymus-bedlylzwhjgke97
ER  -