TY  - JOUR
T1  - Development and Validation of an RP-HPLC Method for Simultaneous Quantification of Azelnidipine and Metoprolol Succinate in Synthetic Mixtures
A1  - Leela Prasad Kowtharapu
A1  - Naresh Kumar Katari
A1  - Siva Krishna Muchakayala
A1  - Surya Prakash Rao Pydimarry
A1  - Vijay Kumar Rekulapally
A1  - Christian A. Sandoval
JF  - Pharmaceutical Sciences and Drug Design
JO  - Pharm Sci Drug Des
SN  - 3062-4428
Y1  - 2022
VL  - 2
IS  - 1
DO  - 10.51847/42JUgzvW13
SP  - 1
EP  - 7
N2  - This study outlines an RP-HPLC technique for quantifying azelnidipine and metoprolol succinate in a synthetic mixture. Azelnidipine, a calcium channel blocker, and metoprolol succinate, a beta-blocker, are both used for the management of hypertension. The method was optimized using a Shimadzu HPLC LC2010 system, equipped with a UV-VIS detector and a binary gradient system. A Hibar ODS C18 5 µm column (250 x 4.6 mm) was used for separation in isocratic mode, with a mobile phase consisting of methanol and water (70:30 v/v, pH 3.0), at a flow rate of 1.0 ml/min and detection set at 230 nm. The method demonstrated linearity for azelnidipine in the range of 8-40 µg/ml and for metoprolol succinate between 25-125 µg/ml. This developed method successfully determined both drugs in synthetic mixtures and followed the ICH Q2 R1 guidelines for method validation.
UR  - https://galaxypub.co/article/development-and-validation-of-an-rp-hplc-method-for-simultaneous-quantification-of-azelnidipine-and-ggcunekxsf07wzl
ER  -