TY  - JOUR
T1  - Enzyme Inhibition and Phytochemical Profiling of Ferula persica Oleo-Gum-Resin Relevant to Alzheimer’s Disease
A1  - Miguel Herrera
A1  - Laura Jimenez
A1  - Andres Vega
JF  - Specialty Journal of Pharmacognosy, Phytochemistry, and Biotechnology
JO  - Spec J Pharmacogn Phytochem Biotechnol
SN  - 3062-441X
Y1  - 2023
VL  - 3
IS  - 1
DO  - 10.51847/r4DDNeCPg7
SP  - 259
EP  - 268
N2  - Numerous medicinal plants have proven valuable in managing Alzheimer’s disease (AD). This research selected the oleo-gum-resin of Ferula persica, referred to as sagapenum, to examine its potential to inhibit enzymes linked to the development and advancement of AD. In addition, a previously unreported phytochemical profile was established. Inhibitory effects of dichloromethane, methanol, and aqueous extracts were evaluated in vitro against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) employing the adapted Ellman’s assay. The most effective extract underwent detailed phytochemical examination via multiple column chromatography approaches. The dichloromethane extract displayed marked selective inhibition of BuChE (IC₅₀ = 23.41 µg/mL), relative to the standard drug donepezil (IC₅₀ = 1.97 µg/mL). Analysis of this extract yielded the isolation and characterization of aurapten, farnesiferol A, umbelliprenin, farnesiferol C, farnesiferone A, karatavicinol, ferocaulidin, and ligupersin A, all of which were screened for cholinesterase (ChE) inhibition. Farnesiferol A proved to be the strongest and most selective BuChE inhibitor (IC₅₀ = 31.46 µg/mL). Furthermore, it demonstrated substantial inhibition of β-secretase 1 (BACE1) (IC₅₀ = 5.14 µM), when benchmarked against the reference compound OM99-2 (IC₅₀ = 0.014 µM), suggesting its potential as a multi-target agent for AD therapy. The pronounced selective BuChE inhibition by the dichloromethane extract of sagapenum, along with the activity of farnesiferol A—particularly its BACE1 suppression—could be instrumental in formulating novel dietary supplements for AD.  
UR  - https://galaxypub.co/article/enzyme-inhibition-and-phytochemical-profiling-of-ferula-persica-oleo-gum-resin-relevant-to-alzheimer-0vfmhtv5kjszu2v
ER  -