TY  - JOUR
T1  - Identification and Extraction of Superoxide-Generating Protein Assemblies from Helianthus tuberosus, Daucus sativus, and Solanum tuberosum
A1  - Ruzanna Simonyan
A1  - Madlena Babayan
A1  - Hasmik Yekmalyan
A1  - Anna Alexanyan
A1  - Gegham Simonyan
A1  - Samvel Alexanyan
A1  - Lilit Darbinyan
A1  - Karen Simonyan
A1  - Maxim Simonyan
JF  - Specialty journal of Pharmacognosy, Phytochemistry, and Biotechnology
JO  - Spec J Pharmacogn Phytochem Biotechnol
SN  - 3062-441X
Y1  - 2023
VL  - 3
IS  - 1
DO  - 10.51847/Vj5MeBCcDs
SP  - 15
EP  - 20
N2  - In this study, isoforms of NPC-Fe(III) complexes were extracted and purified from Helianthus tuberosus (Armenian Jerusalem artichoke), Solanum tuberosum (potato), and Daucus sativus (carrot). For the 1st time, the physicochemical properties of these complexes were determined, including their specific content, O₂⁻-producing activity, steady-state concentration of O₂⁻, and fluorescence intensity. These complexes exhibited peak optical absorbance in both the visible and UV spectra. Hybrid complexes between the isolated NPC-Fe(III) complexes and NADPH oxidase (Nox) were generated, with subsequent observation of O₂⁻ production by these hybrid complexes. Under aerobic conditions, the complexes continually produced O₂⁻ by transferring electrons from NPC to Fe(III), which then reduced O₂ to O₂⁻. Finally, O₂ stabilized the O₂⁻. Overall, the findings suggest that these isoforms of NPC-Fe(III) complexes represent novel prooxidant components from the studied plants, with NPC acting as a bioelectric source for the generation of O₂⁻.
UR  - https://galaxypub.co/article/identification-and-extraction-of-superoxide-generating-protein-assemblies-from-helianthus-tuberosus-hzw9pqisgtepbyu
ER  -