TY  - JOUR
T1  - Investigating the Effects of Astragalus Polysaccharide on Immune Cell Infiltration and Prognostic Biomarker Improvement in Pediatric Acute Myeloid Leukemia
A1  - Anil Deshpande
A1  - Mahesh Joshi
A1  - Rajesh Kulkarni
A1  - Vikram Pawar
A1  - Suresh Patil
JF  - Specialty Journal of Pharmacognosy, Phytochemistry, and Biotechnology
JO  - Spec J Pharmacogn Phytochem Biotechnol
SN  - 3062-441X
Y1  - 2022
VL  - 2
IS  - 1
DO  - 10.51847/Kg0WtkaFEt
SP  - 126
EP  - 141
N2  - Acute myeloid leukemia (AML) is a major form of cancer in children, yet treatment strategies remain limited and outcomes suboptimal. This study explored how Astragalus polysaccharide (APS) might influence immune cell dynamics and prognostic markers in pediatric AML. Gene expression data from the GEO dataset GSE2191 were analyzed to identify differentially expressed genes (DEGs), while APS-associated genes (APSRGs) were retrieved from the Swiss Target Prediction platform. Overlapping DEGs and APSRGs (|logFC| > 1, p < 0.05) were defined as APS-related DEGs (APSRDEGs) and visualized with a Venn diagram. Protein-protein interaction (PPI) networks were constructed to pinpoint key hub genes. Functional enrichment via Gene Ontology (GO) and KEGG pathways was performed to determine the biological roles, molecular functions, cellular locations, and pathways linked to these hub genes. Associations with pediatric AML were further assessed through correlation analysis, ROC curves, and immune infiltration profiling. Comparison between pediatric AML and control samples identified 1,881 DEGs, among which 20 were APSRDEGs. PPI network mapping revealed 13 interconnected APSRDEGs, from which nine hub genes were highlighted: CASP3, PTPRC, ELANE, HMOX1, CHUK, FLT1, JAK3, CTSL, and AURKA. GO and KEGG analyses indicated that these hub genes are involved in critical biological processes and pathways relevant to AML pathogenesis. ROC curve evaluation demonstrated significant differential expression of the hub genes between AML and control groups. Immune infiltration analysis revealed notable correlations between hub gene expression and immune cell presence, with HMOX1 showing the strongest positive association with neutrophils. Nine APS-associated hub genes were identified in pediatric AML, suggesting that APS may modulate immune infiltration and affect disease prognosis. These results point to APS as a promising candidate for therapeutic intervention in pediatric AML.
UR  - https://galaxypub.co/article/investigating-the-effects-of-astragalus-polysaccharide-on-immune-cell-infiltration-and-prognostic-bi-gfr61mzj8bffe7v
ER  -