%0 Journal Article
%T Isolation and Purification of Bioactive Compounds from Endophytic Bacillus sp. RD26 Associated with Phyllanthus Amarus
%A Nguyen Van Minh
%A Nguyen Tan Phat
%A Duong Nhat Linh
%J Specialty journal of Pharmacognosy, Phytochemistry, and Biotechnology
%@ 3062-441X
%D 2021
%V 1
%N 1
%R 10.51847/MZXt8zdCUd
%P 35-42
%X This study investigated the bioactive properties of Bacillus amyloliquefaciens RD26, an endophyte isolated from Phyllanthus amarus. The methanol extract of Bacillus sp. RD26 showed significant antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), with an inhibition zone of 25 ± 0.57 mm. The antibacterial compound, BR04, was isolated and identified as pyrimidine-2,4-dione (uracil), showing potent antimicrobial effects with minimum inhibitory concentrations (MICs) of 64 µg/mL against MRSA, 128 µg/mL against Bacillus cereus, and 512 µg/mL against Escherichia coli. In addition, BR04 showed antioxidant activity and scavenged up to 50% of DPPH free radicals at a concentration of 1800 μg/mL. These findings highlight the potential of BR04 as a bioactive compound for pharmaceutical and agricultural applications, particularly in combating infectious pathogens and providing a natural alternative to synthetic antioxidants.
%U https://galaxypub.co/article/isolation-and-purification-of-bioactive-compounds-from-endophytic-bacillus-sp-rd26-associated-with-4lwktwvvy5pmxrg