Written by Rafael Mendoza
Published on Vol 6 Issue 1, 2026
Drug resistance frequently emerges in breast cancer during chemotherapy, and standalone immunotherapies often yield insufficient results. Therefore, the clinical advancement of combined chemo-immunotherapy strategies is critically needed. However, traditional two-dimensional cell cultures and animal models remain inadequate for reliably assessing the outcomes of such combined treatments. This study presents a straightforward automated multi-well system developed for producing tumor-associated ma
Written by Wouter De Smet
Published on Vol 6 Issue 1, 2026
Preventing sudden cardiac death (SCD) in clinical settings and establishing it accurately in forensic investigations remain difficult because of the lack of uniform quantitative standards for assessing morphological and histopathological changes in the heart. This study aimed to create a machine learning-powered system for evaluating cardiac lesions, thereby enabling more precise quantitative diagnosis and risk assessment of SCD. The investigation incorporated 2284 adult autopsy cases from the f
Written by Ana Rodrigues
Published on Vol 6 Issue 1, 2026
Small non-coding RNAs (ncRNAs) function as bioactive molecules packaged inside extracellular vesicles (EVs), where they influence a broad spectrum of physiological and disease-related pathways. This investigation presents an in-depth expression atlas of seven distinct categories of small ncRNAs in EVs isolated from both serum and plasma across multiple conditions. Large EVs (lEVs) and small EVs (sEVs) alike contain considerable fractions of miRNAs ( ∼ 28.2% in lEVs and ∼ 20.8% in sEVs) and ribos
Written by Krzysztof Wiśniewski
Published on Vol 6 Issue 1, 2026
Nonalcoholic fatty liver disease (NAFLD) represents a spectrum of hepatic disorders that begins with simple steatosis and can advance to nonalcoholic steatohepatitis, potentially progressing further to fibrosis, cirrhosis, and hepatocellular carcinoma. At present, no pharmacological agent has received approval for the treatment of NAFLD-related hepatic steatosis. This situation highlights the urgent requirement for more effective interventions capable of modulating lipid metabolism and preventin
Written by Ahmed Ben Ali
Published on Vol 6 Issue 1, 2026
A growing body of research indicates that PIWI-interacting RNAs (piRNAs) encapsulated in circulating exosomes represent promising novel molecular biomarkers suitable for tumor liquid biopsy. Nonetheless, available techniques for the direct in situ identification of exosomal piRNAs are still inadequate. Here, we developed a spherical nucleic acid-based probe called piR-651 that readily enters exosomes after a 2 h incubation period and permits in situ detection of piR-651 with a limit of detection
Written by Peter Nilsson
Published on Vol 6 Issue 1, 2026
Throughout the COVID-19 pandemic, multiple vaccine platforms were employed across the globe. Nevertheless, the majority depended on immunogenicity data and bridging studies instead of direct retrospective evaluation derived from actual community infections. Consequently, knowledge regarding protective processes during natural exposure to circulating variants remained incomplete. A retrospective cohort analysis based on real-world BA.5 infections was performed to determine the protective performa
Written by James O'Leary
Published on Vol 6 Issue 1, 2026
Accurate excision of orbital tumors continues to present a major surgical challenge. Near-infrared II (NIR-II) fluorescence imaging offers surgeons the possibility of real-time visualization and localization of these tumors. This study represents the first evaluation of the feasibility and potential clinical benefit of NIR-II fluorescence imaging during orbital tumor resection. A dedicated NIR-II fluorescence imaging platform was constructed, with indocyanine green (ICG) utilized as the fluoresc
Written by Lukas Weber
Published on Vol 6 Issue 1, 2026
The development of antibiotic-resistant bacteria represents a major obstacle to the effective and timely management of bacterial infections, including acute bacterial skin and skin-structure infections (ABSSSI), particularly when biofilms are present. Bacterial biofilms exhibit inherent resistance to antibiotics and the host immune response, rendering biofilm-associated infections exceptionally challenging to eradicate. Consequently, the creation of novel antibacterial strategies that specifical
Written by Maria Gonzales
Published on Vol 6 Issue 1, 2026
Disruption of the gut microbiota is a frequent occurrence among patients admitted to intensive care units. Particular shifts, including the proliferation of Enterobacteriaceae and other opportunistic pathogens (pathobionts), have been associated with worse clinical outcomes. In non-critically ill populations, administration of enteral nutrition containing prebiotic short-chain fructooligosaccharides (scFOS-EN) has been shown to help restore microbial balance. The consequences of scFOS-EN supplem
Written by Patrick O'Connor
Published on Vol 6 Issue 1, 2026
The American College of Cardiology and American Heart Association (ACC/AHA) updated their blood pressure (BP) criteria in 2017, establishing lower thresholds to identify hypertension in non-pregnant adults. Adoption of these revised standards in pregnant women might allow earlier recognition of individuals at greater risk for complications. This approach holds particular promise for stratifying risk among first-time mothers. The present secondary analysis of the SCOPE cohort evaluated whether th
Written by Gabriel Costa
Published on Vol 6 Issue 1, 2026
Long non-coding RNAs (lncRNAs) exert essential regulatory effects on the activation and phenotypic conversion of cancer-associated fibroblasts (CAFs). Nevertheless, their involvement has yet to be thoroughly explored using single-cell approaches. Leveraging comprehensive integrated single-cell transcriptomic datasets, we identified lncRNAs with abnormal expression patterns specifically within CAFs, the primary cellular element of the tumor microenvironment. Our analysis uncovered a pan-cancer pa
Written by Luca Ferraro
Published on Vol 6 Issue 1, 2026
Circulating tumor cells (CTCs) provide an essential foundation for core cancer research and for reliably assessing the outcomes of medical treatments. Yet, recovering CTCs from patient blood samples remains technically demanding, with the main obstacles stemming from limitations in biomarker selectivity and the overall sensitivity of isolation procedures. In an earlier investigation, we introduced an innovative detection strategy for CTCs centered on a defining biophysical property of cancer cel
Written by Pieter Botha
Published on Vol 6 Issue 1, 2026
Phenotypic modulation of smooth muscle cells (SMC) constitutes a central mechanism contributing to the development of pulmonary arterial hypertension (PAH). Despite its importance, the regulatory pathways involved are not yet fully understood. In this study, single-cell RNA sequencing was applied to pulmonary arterial tissues procured from lung transplant recipients to characterize cellular diversity and the transcriptomic signatures of major cell populations. Three separate SMC subpopulations w
Written by Santiago Morales
Published on Vol 6 Issue 1, 2026
CCTA has become a standard noninvasive imaging technique for the initial diagnosis and clinical management of coronary artery disease (CAD). Advanced machine learning techniques applied to CCTA data demonstrate considerable potential for precise quantitative evaluation of CAD. In a post hoc study involving 909 subjects from the SCOT-HEART trial (with a median follow-up period of 5.8 years), we initially examined the variability of CCTA-based imaging parameters in a reference group of 221 individ
Written by Nora Schneider
Published on Vol 6 Issue 1, 2026
Latest findings stress how vital the nervous system is for tumors to advance. Still, knowledge about the specific ways nerves affect the start and growth of stomach cancers stays quite incomplete. This project intends to uncover shifts in nerve presence, the main genes connected to them, and the key signaling routes active while gastric tumors form. We included 257 people who had gastric precancerous lesions (GPLs) drawn from four separate national patient groups. Using immunohistochemistry stai
Written by Daniel Kim
Published on Vol 6 Issue 1, 2026
Chimeric antigen receptor (CAR) T-cell therapy has become a key treatment for blood cancers thanks to its strong capacity to identify and destroy malignant cells. Evaluating the performance of these immune cells at the individual level is essential for optimizing diagnostic and therapeutic outcomes. There is a pressing need for a versatile, rapid, high-throughput, and comprehensive system to assess immune cell efficacy. In this work, the killing ability, expansion capacity, and long-term surviva
Written by Mateo Alvarez
Published on Vol 6 Issue 1, 2026
Ovarian cancer exhibits the highest death rate among gynecological cancers and is typically first evaluated through ultrasound. However, the intricate nature of ultrasound visuals for ovarian lesions, combined with the deep pelvic positioning, demands substantial expertise for accurate subjective interpretation. Consequently, identifying ovaries and ovarian lesions, along with diagnosing ovarian cancer, remains difficult. This study sought to create an automated deep learning-based system called
Written by Sara Ben Youssef
Published on Vol 6 Issue 1, 2026
Modern paediatric cancer management needs improved methods for forecasting patient outcomes to enable tailored risk grouping. However, research evaluating the effectiveness, makeup, and shortcomings of current prognostic tools remains limited. This study sought to evaluate the predictive performance of conventional and more sophisticated prognostic approaches. This systematic review and meta-analysis (registered as CRTN42022370251) involved a comprehensive literature search across PubMed, Embase
Written by Noah Williams
Published on Vol 6 Issue 1, 2026
Manual evaluation of tumor-infiltrating lymphocytes (TILs) by pathologists has revealed important predictive and prognostic roles in both early-stage and advanced triple-negative breast cancer (TNBC). Despite this, the approach continues to suffer from inconsistency. Artificial intelligence (AI) represents an effective way to minimize such inconsistency and support fully automated, impartial TILs measurement. The main hurdle to widespread clinical use is proving strong analytical and prognostic
Written by Elena Petrova
Published on Vol 6 Issue 1, 2026
Changes in infant alertness and neurological status may indicate serious underlying conditions, yet these are typically evaluated through physical examinations that occur intermittently and involve subjective judgment. There is a clear need for dependable, ongoing monitoring techniques. We proposed that a computer vision technique using pose estimation artificial intelligence (AI) to monitor movement could help forecast neurological alterations in the neonatal intensive care unit (NICU). Video r
Written by Lina Hassan
Published on Vol 6 Issue 1, 2026
Although autoimmunity driven by dendritic cells (DCs) and CD8+ T cells plays a central role in the destruction of melanocytes during vitiligo, effective treatments are scarce due to the lack of approaches that simultaneously address both cell populations. We investigated the link between the immunoregulatory metabolite itaconate and vitiligo progression by measuring serum itaconate concentrations in patients with vitiligo and tracking depigmentation in Acod1 knockout (KO) mice, which lack endoge
Written by Saif Al-Hinai
Published on Vol 6 Issue 1, 2026
The purpose of this investigation was to determine the added value of metabolic imaging, perfusion mapping, and diffusion kurtosis imaging (DKI) in accurately identifying and forecasting outcomes for patients with H3K27-altered diffuse midline gliomas (DMGs). A total of 95 individuals (mean age 16.98 years; 61.1% female) with brainstem masses were enrolled between June 2020 and May 2023. This cohort included 71 patients with H3K27-altered DMGs and 24 patients harboring H3K27 wild-type brainstem
Established in 2019, Galaxy Publication stands as a global academic publishing house focused on advancing scholarly work across medicine, nursing, and health sciences. Through its network of peer-reviewed journals, the organization brings together original research and academic contributions from scholars around the world, creating a shared space for knowledge exchange and intellectual collaboration.
