Volume 6, Issue 1 (2026)

Drug resistance frequently emerges in breast cancer during chemotherapy, and standalone immunotherapies often yield insufficient results. Therefore, the clinical advancement of combined chemo-immunotherapy strategies is critically needed. However, traditional two-dimensional cell cultures and animal models remain inadequate for reliably assessing the outcomes of such combined treatments. This study presents a straightforward automated multi-well system developed for producing tumor-associated ma

Preventing sudden cardiac death (SCD) in clinical settings and establishing it accurately in forensic investigations remain difficult because of the lack of uniform quantitative standards for assessing morphological and histopathological changes in the heart. This study aimed to create a machine learning-powered system for evaluating cardiac lesions, thereby enabling more precise quantitative diagnosis and risk assessment of SCD. The investigation incorporated 2284 adult autopsy cases from the f

Small non-coding RNAs (ncRNAs) function as bioactive molecules packaged inside extracellular vesicles (EVs), where they influence a broad spectrum of physiological and disease-related pathways. This investigation presents an in-depth expression atlas of seven distinct categories of small ncRNAs in EVs isolated from both serum and plasma across multiple conditions. Large EVs (lEVs) and small EVs (sEVs) alike contain considerable fractions of miRNAs ( ∼ 28.2% in lEVs and ∼ 20.8% in sEVs) and ribos

Nonalcoholic fatty liver disease (NAFLD) represents a spectrum of hepatic disorders that begins with simple steatosis and can advance to nonalcoholic steatohepatitis, potentially progressing further to fibrosis, cirrhosis, and hepatocellular carcinoma. At present, no pharmacological agent has received approval for the treatment of NAFLD-related hepatic steatosis. This situation highlights the urgent requirement for more effective interventions capable of modulating lipid metabolism and preventin

A growing body of research indicates that PIWI-interacting RNAs (piRNAs) encapsulated in circulating exosomes represent promising novel molecular biomarkers suitable for tumor liquid biopsy. Nonetheless, available techniques for the direct in situ identification of exosomal piRNAs are still inadequate. Here, we developed a spherical nucleic acid-based probe called piR-651 that readily enters exosomes after a 2 h incubation period and permits in situ detection of piR-651 with a limit of detection

Throughout the COVID-19 pandemic, multiple vaccine platforms were employed across the globe. Nevertheless, the majority depended on immunogenicity data and bridging studies instead of direct retrospective evaluation derived from actual community infections. Consequently, knowledge regarding protective processes during natural exposure to circulating variants remained incomplete. A retrospective cohort analysis based on real-world BA.5 infections was performed to determine the protective performa

Accurate excision of orbital tumors continues to present a major surgical challenge. Near-infrared II (NIR-II) fluorescence imaging offers surgeons the possibility of real-time visualization and localization of these tumors. This study represents the first evaluation of the feasibility and potential clinical benefit of NIR-II fluorescence imaging during orbital tumor resection. A dedicated NIR-II fluorescence imaging platform was constructed, with indocyanine green (ICG) utilized as the fluoresc

The development of antibiotic-resistant bacteria represents a major obstacle to the effective and timely management of bacterial infections, including acute bacterial skin and skin-structure infections (ABSSSI), particularly when biofilms are present. Bacterial biofilms exhibit inherent resistance to antibiotics and the host immune response, rendering biofilm-associated infections exceptionally challenging to eradicate. Consequently, the creation of novel antibacterial strategies that specifical