Written by Haruto Watanabe
Published on Vol 5 , 2025
Prolonged exposure to glucocorticoids frequently results in glucocorticoid-induced osteoporosis (GIOP). This work investigated whether eldecalcitol (ED-71), a new active analog of vitamin D3, can counteract GIOP and sought to clarify the molecular pathways responsible for its protective action. A GIOP mouse model was generated by administering methylprednisolone (MPED) or dexamethasone (DEX) intraperitoneally to C57BL/6 mice, while ED-71 was concurrently delivered by oral gavage. Bone morphology
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Written by Isabel Romero
Published on Vol 5 , 2025
Depression is a common complication in Chronic Kidney Disease (CKD) that significantly impacts patient prognosis. Modified Danggui-Shaoyao-San (MDSS), an optimized version of the traditional Chinese formula Danggui-Shaoyao-San (DSS), has been developed for depression management. This study aimed to assess the clinical efficacy and safety of MDSS in treating depression in CKD patients and to explore its underlying molecular mechanisms using pharmacological analysis and molecular docking. A total
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Triptolide, a principal bioactive compound derived from Tripterygium wilfordii Hook f., has shown efficacy in treating membranous nephropathy (MN), yet its precise pharmacological mechanisms remain unclear. This study employed an integrated approach combining network pharmacology and experimental validation to systematically elucidate the molecular mechanisms underlying triptolide’s therapeutic effects in MN. Potential targets of triptolide and MN-associated targets were retrieved from publicly
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Written by Elias Njoroge
Published on Vol 5 , 2025
This study aimed to uncover the mechanisms by which Agrimonia pilosa Ledeb. (APL) extract mitigates acute myocardial infarction (AMI) using a combination of network pharmacology and experimental validation. The main bioactive constituents of APL extract were identified through High-Performance Liquid Chromatography (HPLC). Potential targets shared between APL compounds and AMI were predicted via network pharmacology. An AMI model in mice was induced by subcutaneous isoproterenol (Iso) injection,
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Written by Santiago Flores
Published on Vol 5 , 2025
Longdan Xiegan Decoction (LXD) is a well-known traditional Chinese herbal formula with demonstrated inhibitory effects on inflammatory cells involved in uveitis. However, the principal functional component combinations and their underlying mechanisms remain unclear.To identify the key component group (KCG) and explore the potential mechanisms of LXD in uveitis treatment, we applied a network community detection model, functional response space analysis, and a reverse prediction model. The effect
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Imrecoxib is a novel, moderately selective cyclooxygenase-2 (COX-2) inhibitor with anti-inflammatory and analgesic properties, commonly used in patients with osteoarthritis. However, pharmacokinetic data to guide its use in the elderly are currently lacking.This study aimed to evaluate the pharmacokinetics of imrecoxib and its primary metabolites and to assess its safety in healthy elderly subjects.Nineteen healthy participants, including 10 non-elderly and 9 elderly individuals, received a sing
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Written by Olivia Turner
Published on Vol 5 , 2025
Chaihu Shugan San (CSS), a classical formula long employed in the management of major depressive disorder (MDD), has repeatedly demonstrated both efficacy and safety in clinical settings. Impaired angiogenesis has emerged as a critical component of MDD pathology, yet the specific mechanisms by which CSS influences angiogenic processes remain insufficiently defined. To clarify these mechanisms, network pharmacology was first used to map potential angiogenesis-related targets and pathways linking
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Written by Johanna Eriksson
Published on Vol 5 , 2025
Semaglutide, a novel long-acting glucagon-like peptide-1 (GLP-1) analogue, has demonstrated therapeutic benefits in renal diseases; however, its specific effects on kidney metabolism in the context of obesity remain unclear. This study aimed to investigate the renoprotective effects of semaglutide and its underlying metabolic regulatory mechanisms in obesity-associated kidney injury. Male C57BL/6J mice were assigned to control and obesity groups, with the latter receiving a high-fat diet and tre
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This study aimed to investigate microsponges (MS) as a drug delivery system using Adapalene (ADA) as the model compound.A Plackett-Burman design was employed to identify the critical factors influencing ADA-MS formulation. Microsponges were prepared via the quasi-emulsion solvent diffusion technique. The independent variables examined included organic phase volume, sonication duration, stirring speed, drug loading percentage, polymer type, emulsifier concentration, and the method of organic phas
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This study aimed to explore potential relationships between gut microbiota composition and hepatic metabolic disturbances in mice with methotrexate (MTX)-induced hepatotoxicity.Hepatotoxicity was induced in healthy Kunming mice using MTX, followed by measurements of plasma ALT and AST levels and histopathological examination of liver tissue. An integrated approach combining gas chromatography–mass spectrometry (GC-MS) and 16S rRNA gene sequencing was employed to assess MTX-induced alterations in
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Written by Amanda Brooks
Published on Vol 5 , 2025
Osteoporosis is a bone disorder marked by reduced bone density and increased fracture risk, primarily due to enhanced formation or activity of bone-resorbing osteoclasts. Although current anti-resorptive therapies can effectively reduce osteoclast-mediated bone loss, their long-term use is often limited by adverse effects. Pristimerin (PRI), a naturally occurring quinone-methide triterpenoid, has been reported to possess anti-inflammatory and anti-cancer properties through modulation of signalin
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Written by Sara Lindholm
Published on Vol 5 , 2025
Sodium cantharidinate (SC) has demonstrated efficacy in treating lung cancer in China, yet its effects in cervical cancer (CC)—a major cause of female reproductive cancer mortality—remain largely unexplored. This study investigates SC’s anti-cancer activity in CC and explores the molecular mechanisms involved. DDP-resistant Caski-1 and ME180 cervical cancer cell lines were established and treated with SC to assess its impact on cell proliferation. Potential molecular targets of SC were predicted
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Written by Vlad Stanescu
Published on Vol 5 , 2025
Pre-B cell colony enhancing factor (PBEF) is recognized as a proinflammatory cytokine involved in acute lung injury, but its contribution to lung damage following cardiopulmonary bypass (CPB) has not been clarified. This study explored how reducing PBEF expression affects lung injury and the regulation of sodium and water transport in a rat model of CPB. Lung morphology was examined using hematoxylin and eosin staining. Immunohistochemistry was applied to assess PBEF levels. Western blotting was
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Written by Mohamed Abdelrahman
Published on Vol 5 , 2025
Doxorubicin is widely used for cancer therapy but is limited by its cardiotoxic effects. Traditional approaches, such as lowering the drug dose, aim to reduce cardiac side effects but may compromise anticancer efficacy. This study investigates whether Roflumilast can protect heart cells from Doxorubicin-induced inflammation and premature cellular aging, and explores the role of SIRT1 in this process. H9c2 cardiomyocytes were exposed to 5 μmol/L Doxorubicin to induce cell injury. Cell survival wa
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Evidence from clinical trials such as INPULSIS-ON indicates that extended use of nintedanib is generally safe for patients with idiopathic pulmonary fibrosis (IPF). Nevertheless, real-world data on long-term therapy are scarce. This study examined how effective and tolerable prolonged nintedanib treatment is when used in everyday clinical care. This retrospective review included 104 individuals diagnosed with IPF who received nintedanib. Patients were divided into two cohorts: those who maintain
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Cisplatin (DDP) remains a primary chemotherapeutic agent for treating non-small cell lung cancer (NSCLC), but the development of drug resistance limits its clinical efficacy. This study aimed to explore the role of RASSF1A in modulating DDP resistance in NSCLC and to elucidate the underlying molecular mechanisms. Expression levels of RASSF1A and microtubule-associated protein 1S (MAP1S) were assessed using qRT-PCR and Western blotting, while their interaction was verified by co-immunoprecipitati
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Written by Carlos Mendes
Published on Vol 5 , 2025
This research investigated whether Lactobacillus plantarum KSFY06 (LP-KSFY06) could prevent acute liver damage caused by combined D-galactose and lipopolysaccharide (D-Gal/LPS) exposure in mice. The antioxidant activity of LP-KSFY06 was first examined using in vitro radical scavenging assays. In mice, the effects on liver injury were assessed through organ weight indices, serum liver function markers, oxidative stress parameters, inflammatory cytokines, gene expression levels, and histological e
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Written by Yuki Nakamura
Published on Vol 5 , 2025
Effective ocular delivery of tacrolimus remains challenging due to rapid clearance and low bioavailability. This study aimed to design an in situ gel incorporating tacrolimus-loaded solid lipid nanoparticles (TAC-SLNs) for improved therapeutic outcomes in immune conjunctivitis. The TAC-SLNs in situ gel was optimized and evaluated for particle morphology, size, surface charge, drug encapsulation efficiency, loading capacity, and release profile in vitro. Pharmacokinetic and pharmacodynamic studie
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