This study evaluated the impact of an educational intervention using short message service (SMS) and WhatsApp messages on the tuberculosis (TB) knowledge of pharmacy personnel. A prospective study was conducted with a random sample of pharmacies and drugstores in two districts of Lima and Chiclayo. The intervention included the development and pilot testing of text messages, content evaluation, selection of participating pharmacies, implementation, and a post-intervention survey to assess changes in TB knowledge. A total of 132 participants completed the final survey, with a median age of 27 years (IQR: 23–30) and 78.46% being female. At baseline, average correct responses were 66.33% for epidemiology, 62.22% for diagnosis, and 54.44% for treatment, with only 45.45% scoring above the 55th percentile. After the intervention, knowledge on TB prevention significantly improved (72.55 vs. 66.33, p = 0.027) among those who read the messages, and overall TB knowledge was higher in this group (p = 0.034). In conclusion, while pharmacy staff had generally adequate TB knowledge, notable gaps remained in treatment-related understanding, which were partially addressed through the SMS-based educational intervention.
