A straightforward and highly sensitive isocratic reversed-phase HPLC technique has been developed for the detection and quantification of ciprofloxacin in pharmaceutical formulations, which has been validated following ICH guidelines. This study introduces a straightforward, cost-effective HPLC technique that delivers accurate results, a low limit of quantification (LOQ), and reduced analysis time. The separation process was performed on a C18 reversed-phase column, using a mobile phase of acetonitrile and water in a ratio of 80:20 and with a pH adjustment of 2.7 achieved by the addition of 85% phosphoric acid. Ultraviolet detection occurred at 275.0 nm, with a flow rate of 1.7 mL/min at ambient temperature. Statistical evaluations of the precision, both inter- and intra-day, showed that the method is highly accurate and precise, with a correlation coefficient of 0.9999 or higher. The detection limit was 500 ng/mL, and the recovery was found to range between 99.01% and 101.19%. This method is reliable for routine analysis in pharmaceutical and clinical laboratories and can also be used for the measurement of ciprofloxacin in human serum.
