Pharmacogenomics (PGx) focuses on using genetic information to improve and personalize drug therapy. Many clinical centers have already begun incorporating pharmacogenetic testing into routine care. Next-generation sequencing (NGS) is becoming a more comprehensive, efficient, and cost-effective tool for PGx applications. This review outlines the key factors involved in using NGS to guide medication decisions in clinical settings. It examines both the benefits and the challenges associated with adopting NGS-based PGx testing. In addition, it describes the limitations of different NGS platforms and highlights practical strategies for establishing and managing these technologies in clinical practice.
