Despite its potential, the integration of pharmacogenomics (PGx) testing into clinical practice remains limited, largely due to gaps in education and training. This study investigates healthcare professionals’ (HCPs) knowledge, attitudes, and confidence in applying PGx, using surveys conducted before and after a structured training program. The training program was organized into five modules addressing three main learning goals. Participants completed surveys both prior to and following the training to assess changes in their self-perceived ability and readiness to implement PGx in clinical settings. Among 102 respondents, most acknowledged the value of PGx testing, yet many reported low confidence and skill in utilizing PGx data. Post-training results demonstrated a significant improvement in self-efficacy and competency, indicating that brief, focused educational interventions can enhance preparedness and may encourage broader adoption of PGx in healthcare practice.