Research has shown that the extract from Wedelia chinensis improves the outcomes of prostate cancer therapy. This investigation examined genes with altered expression in the 22RV1 prostate cancer cell line exposed to W. chinensis extract, utilizing data retrieved from the Gene Expression Omnibus (GEO), followed by gene ontology and pathway enrichment analyses. Expression data from the series GSE100224 were evaluated with GEO2R. Key genes showing differential expression were investigated through interaction mapping. Associated biological functions and processes linked to these genes were determined. Prominent disrupted genes and pathways were reviewed in detail. A total of seventy key differentially expressed genes, consisting of 49 upregulated and 21 downregulated, were analyzed for interactions involving inhibition, activation, expression, and binding. Cytochrome P450 and PTGS2 stood out as critical genes. The pathway primarily affected was estrogen metabolism. The evidence points to “estrogen metabolism” as the chief pathway influenced by W. chinensis in 22RV1 cells, with UGT1A1, MAOA, PTGS2, and cytochrome P450 being the main genes involved.
