This work aimed to investigate both the antibacterial potential and the phytochemical composition of six medicinal plants long utilized in local traditional remedies for various illnesses. Methanol-based extracts from these species were evaluated for antimicrobial performance through Agar well diffusion assays and Microtiter broth dilution techniques. Among the tested materials, the root extract of Andrachne aspera produced the largest inhibition zones against S. epidermidis, showing statistically significant values (p < 0.05) of 33 ± 0.17 at 100 mg/ml and 33.5 ± 0.84 at 200 mg/ml. The next strongest activity was observed from Dichrostachys cinerea leaf extract at 200 mg/ml, reaching 24.8 ± 0.41 against the same bacterium. Minimum inhibitory concentration results included 1.0 ± 0.0 for Andrachne aspera against E. faecalis, while Dichrostachys cinerea registered 2.0 ± 0.0 against S. aureus. Phytochemical screening indicated that both species were rich in Polyphenols and Flavonoids. These findings highlight the need for further in-depth research, including safety evaluations, structural analyses, and isolation of active secondary metabolites with potential application as new antimicrobial drugs.
